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Product | Size | Cat.No. | Price（￥） |
BugBuster® Protein Extraction Reagent | 100 ml | 70584-3 | 750.00 |
500 ml | 70584-4 | 3,789.00 | |
BugBuster® Master Mix | 100 ml | 71456-3 | 1,700.00 |
500 ml | 71456-4 | 5,200.00 | |
CytoBuster™ Protein Extraction Reagent | 50 ml | 71009-3 | 824.00 |
250 ml | 71009-4 | 3,122.00 |
 | Comparison of E. coli lysis methods 50 ml samples of an induced 500 ml culture of BL21（DE3） containing pET-41a（+） encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity. |
Product | Size | Cat.No. | Price（￥） |
Benzonase® Nuclease, Purity > 90% | 10 ku | 70746-3 | 1,200.00 |
2.5 ku | 70746-4 | 400.00 |
 | Viscosity reduction by Benzonase Nuclease E. coli BL21（DE3） cells containing a pET construct were suspended in BugBuster Reagent （5 ml/g wet weight）. Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at |
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