世界*實(shí)驗(yàn)材料供應(yīng)商 Dianova 正式授權(quán)上海起發(fā)為其中國代理， Dianova 在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務(wù)，簽約 Dianova 就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲

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Dianova公司為德國的病理免疫組化產(chǎn)品供應(yīng)商，具備的抗體制備工藝及嚴(yán)格的質(zhì)控系統(tǒng)，產(chǎn)品在上享有良好的聲譽(yù)。

克隆號(hào)為H09的IDH1（isocitrate dehydrogenase 1, 異檸檬酸鹽脫氫酶1）鼠單克隆抗體為Dianova公司近期推出的產(chǎn)品之一，此抗體可特異性的檢測腦組織腫瘤中IDH1基因的R132H點(diǎn)突變。IDH1基因的第132密碼子的雜合突變是II 和III級(jí)神經(jīng)膠質(zhì)瘤（根據(jù)WHO的腫瘤學(xué)分類）的頻發(fā)事件。在大約70％的星形膠質(zhì)細(xì)胞瘤和少突神經(jīng)膠質(zhì)細(xì)胞瘤可發(fā)現(xiàn)IDH1基因的R132H點(diǎn)突變。

IDH1基因R132H突變?cè)诓糠帜X腫瘤中的頻發(fā)現(xiàn)象使克隆號(hào)為H09的IDH1抗體可高靈敏性和高特異性的用于此類腫瘤的鑒別診斷中：如從原發(fā)性膠質(zhì)母細(xì)胞瘤中區(qū)分出間變性星形細(xì)胞瘤、從毛細(xì)胞型星形細(xì)胞瘤或室管膜（細(xì)胞）瘤中區(qū)分出彌漫性星形細(xì)胞瘤（WHO分類II級(jí)），也可以用于具有IDH1突變的腫瘤細(xì)胞浸潤前沿的與反應(yīng)性神經(jīng)膠質(zhì)增多癥的區(qū)分。因此，由于其高靈敏性和高特異性，此抗體在腦部腫瘤的分類分級(jí)中以及在檢測單一或少量浸潤腫瘤細(xì)胞時(shí)是非常有用的。

DIA-303 – Anti-CD3 e (Ms) aus Ratte (Klon: HH3E) für Paraffingewebe aus Maus – 500 µl

Art.-Nr.                        DIA-303

Spezifität                      CD3 e

Spezies-Reaktivität             Maus

Wirtsspezies                     Ratte

Isotyp                           IgG1/k

Klon                             HH3E

Klonalität (Mono-/Polyklonal)     monoklonal

Anwendung                        Immunhistochemie (IHC)

                                 Immunhistochemie (Paraffingewebe)
                                 Gefrierschnitte (IHC-F)
                                 Western Blot

Konjugation                       unkonjugiert

Verdünnung                        Histo-/Zytochemie 1:100

Format                            Lyophilisat

                                  gereinigter Antikörper (aus Kulturüberstand)

Produktlinie / Thema               CD-Marker

                                   Immunhistochemie

Zweckbestimmung                     nur für Forschungszwecke

Suchcode                             DIA303M

                                     DIA303

Hersler / Marke                   dianova

Reactivity:

Antibody clone HH3E has been validated specifically for the detection of murine CD3 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). It detects a conserved epitope on the CD3 epsilon chain in a broad variety of species. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.
Cluster of differentiation 3 (CD3) is composed of four distinct polypeptide chains CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta, that form a multimeric protein complex. The CD3 complex associates non-covalently with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal trans-duction molecules. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into in-tracellular signals that elicit a change in the cell from a dormant to an activated state. During T cell maturation the expression of CD3 migrates from the cytoplasm of pro-thymocytes to the cell-membrane of thymocytes. The specific appearance at all stages of T cell development make CD3 an ideal marker for normal T cells and T cell neoplasms (lymphomas, leukemias). Moreover, CD3 is a usefull immunohistochemical marker for T cells in tissue sections.
In a clinical setting in humans, CD3 serves as an important T cell marker for the classification of malignant lymphomas and leu-kemias. It can also be used to detect T cells in coeliac disease, lymphocytic and collagenous colitis. An anti-CD3 epsilon anti-body (Okt3) has been clinically approved for the induction of immunosuppression in organ transplantation. In animal studies anti-CD3 antibodies can induce tolerance to allografts.

Immunohistochemistry of mouse CD3e (TCRE) in formalin-fixed paraffin-embedded tissue sections

The monoclonal antibody clone HH3E specifically stains mouse tissue sections by IHC-FFPE: Lymph nodes (A,B), spleen (C) and kidney (D).

All sections were stained by an indirect alkaline phosphatase method according to standard procedures with antigen retrieval by high-temperature heating in citrate buffer and counterstaining with Haematoxylin.

A

B

C

D

Related References:

1. Leon F. Flow cytometry of intestinal intraepithelial lymphocytes in celiac disease. Journal of Immunological Methods 363: 177-186, 2011
2. Smith-Garvin JE et al.. T cell activation. Annu Rev Immunol. 27:591-619, 2009
3. Sapp H et al.The terminal ileum is affected in patients with lymphocytic or collagenous colitis. Am J Surg Pathol. 26(11):1484-1492, 2002
4. Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clon-ality by polymerase chain reaction. Vet Immunol Immunopathol. 69:145-164, 1999
5. Mosnier et al. Lymphocytic and collagenous colitis: an immunohistochemical study. Am J Gastroenterol. 91(4):709-713, 1996
6. Chetty R, Gatter K. CD3: Structure, function, and role of immunostaining in clinical practice. The Journal of Pathology 173(4): 303–307, 1994
7. Salvadori S et al. Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors. J Immunol. 153(11):5176-5182, 1994
8. Nicolls MR et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation 55(3):459-468, 1993

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